| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217924 |
| Chromosome: | chromosome 4 |
| Location: | 3680652 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228925 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCATGGTGGTGGGCTTGCCGATGATGT |
| Internal bar code: | CTTCAGAATCTACGTCTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 58.341 |
| LEAP-Seq n confirming: | 633 |
| LEAP-Seq n nonconfirming: | 452 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCACCACAGCAGCAATA |
| Suggested primer 2: | TTGTCTTACATCGCCTGTGC |