| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217924 |
| Chromosome: | chromosome 13 |
| Location: | 4210893 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592500 | (1 of 1) K00953 - FAD synthetase (FLAD1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGAGCTATGGATAGCGGGGGGAGGGT |
| Internal bar code: | GTTGGCAGTGCTGGCAGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 792 |
| LEAP-Seq percent confirming: | 99.845 |
| LEAP-Seq n confirming: | 2576 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTTCCCCATCCTCAAGA |
| Suggested primer 2: | TCAGAAATGACGAGCTGTGG |