| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217935 |
| Chromosome: | chromosome 2 |
| Location: | 2547625 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092700 | FAP45 | Flagellar Associated Protein 45; (1 of 1) PTHR15504:SF0 - COILED-COIL DOMAIN-CONTAINING PROTEIN 19, MITOCHONDRIAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTCTTTATTTGGAGGTAGCAAGCGGAC |
| Internal bar code: | TTTCAATGGATTCCCGGTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.4031 |
| LEAP-Seq n confirming: | 7994 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACCTTGTGATGTGGCA |
| Suggested primer 2: | GGTATAATGCGGCGTTCTGT |