| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217935 |
| Chromosome: | chromosome_2 |
| Location: | 2547626 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g092700 | FAP45 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCTACATAGCTCTGCTTTCCGGAGCGGTA |
| Internal bar code: | GAGGTTGCGGGAGTCCCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 893 |
| LEAP-Seq percent confirming: | 98.9987 |
| LEAP-Seq n confirming: | 9689 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAACCTTGTGATGTGGCA |
| Suggested primer 2: | GGTATAATGCGGCGTTCTGT |