| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217941 |
| Chromosome: | chromosome 9 |
| Location: | 4138437 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393802 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGAAGAGAGGATGACAAGCGATACAGA |
| Internal bar code: | GGTGGAGATCATAGCTTGATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 98.8699 |
| LEAP-Seq n confirming: | 4637 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCCGCCCAAGAATAAGC |
| Suggested primer 2: | CCCGGCTCAAAGATACAGAG |