| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217963 |
| Chromosome: | chromosome 16 |
| Location: | 6769666 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673841 | (1 of 1) PTHR13481:SF0 - UPF0454 PROTEIN C12ORF49 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGGCACAACATCAGATGCTGCTCCT |
| Internal bar code: | AGGGCGGAAACCGAGAGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 99.7227 |
| LEAP-Seq n confirming: | 3596 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGACATTGGCATAACAG |
| Suggested primer 2: | CTTCGTCTGTCATTGCCTCA |