| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217993 |
| Chromosome: | chromosome 5 |
| Location: | 322055 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233300 | (1 of 1) 2.1.1.98 - Diphthine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTACGTTCCCCTTGGTACGCAGCTCCC |
| Internal bar code: | TTTGTTTTAGTACTCCTGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 99.3103 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTGTGGAATTGTGTG |
| Suggested primer 2: | CGTCGTACCACACCTTGTTG |