| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217998 |
| Chromosome: | chromosome_16 |
| Location: | 750005 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g691000 | EFP1 | Organellar elongation factor P | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAAGACCACAAGCTCCGGATCCTTGCTTT |
| Internal bar code: | GAGTTAAGGCACGTCCTTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.5124 |
| LEAP-Seq n confirming: | 16328 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGGTTATGGGCTTATGG |
| Suggested primer 2: | GTTGTTGTGCATGGCATAGG |