| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217998 |
| Chromosome: | chromosome 16 |
| Location: | 750216 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691000 | EFP1 | Organellar elongation factor P; (1 of 1) K02356 - elongation factor P (efp) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGTACGGCTGGAGGACGTAGGAGGCAT |
| Internal bar code: | CCGACTCCTTGGTGAGTATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 99.4744 |
| LEAP-Seq n confirming: | 25550 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCATCAAGGTCGACACC |
| Suggested primer 2: | GTTGTTGTGCATGGCATAGG |