| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218018 |
| Chromosome: | chromosome 12 |
| Location: | 1644044 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486209 | (1 of 1) K09533 - DnaJ homolog subfamily C member 13 (DNAJC13) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCGCATGAACGAACATACGGATGTAAC |
| Internal bar code: | AAGCGACGACGGCCCCGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 99.789 |
| LEAP-Seq n confirming: | 1892 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGGTTCGCATGTTTTTAG |
| Suggested primer 2: | GGCTGGCGGACTATGTGTAT |