| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218040 |
| Chromosome: | chromosome 1 |
| Location: | 2811302 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016600 | PSBS1 | (1 of 2) K03542 - photosystem II 22kDa protein (psbS); chloroplast Photosystem II-associated 22 kDa protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGATGCACAAGAGAGCGAAGGATAGG |
| Internal bar code: | CAGTTTCGTTTAGTTTCGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 94.159 |
| LEAP-Seq n confirming: | 6158 |
| LEAP-Seq n nonconfirming: | 382 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGTATATGGGAGTGAGT |
| Suggested primer 2: | CTTCACCAAGGAGAACGAGC |