| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218047 |
| Chromosome: | chromosome 5 |
| Location: | 362448 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233305 | (1 of 1) K07071 - uncharacterized protein (K07071) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATAGAGCGTGTTAATAGGAGGTGTTG |
| Internal bar code: | GGCTGCGCTTGGTTTTATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 70.7865 |
| LEAP-Seq n confirming: | 567 |
| LEAP-Seq n nonconfirming: | 234 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAAGCTCTTCACCCAC |
| Suggested primer 2: | AACCGTTACAGGATAGGGGG |