| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218084 |
| Chromosome: | chromosome_9 |
| Location: | 3666810 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390578 | FAP11 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTGGCGTGCATGTAGTCCGGCCGGGCGAA |
| Internal bar code: | TTAGCGGAACAGTGGCCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1078 |
| LEAP-Seq percent confirming: | 98.372 |
| LEAP-Seq n confirming: | 4834 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAAGGCATTAGCTGAGT |
| Suggested primer 2: | CCCGTCCTCGTGTCTGTTAT |