| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218096 |
| Chromosome: | chromosome 12 |
| Location: | 7670691 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554300 | NSS6 | Sodium:solute symporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGTGTTGGGCGGCGCGCAGGCCTGTGA |
| Internal bar code: | TGGGTTCAATTTATGGTATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 78.75 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACTACGAGGGCATGAGG |
| Suggested primer 2: | ATGTTTTGTTGGGGATGGAA |