| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218106 |
| Chromosome: | chromosome 4 |
| Location: | 3063090 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225250 | (1 of 3) PF14494 - Domain of unknown function (DUF4436) (DUF4436) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCCCAGAAACCAGGTGTTGGAGGGCCC |
| Internal bar code: | GCGAGTAAGTGGAAGTTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 99.3056 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGTCACTTCTCGCTTCA |
| Suggested primer 2: | GTGCGACCCCATGAGTAAGT |