Insertion junction: LMJ.RY0402.218109_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.218109
Chromosome:chromosome 13
Location:4170865
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g592100 (1 of 1) PF00097//PF00628 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // PHD-finger (PHD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCATCGTGTGCTGTTTGTTGCGCCAAGC
Internal bar code:CAGCGCTTCGCGGGGATGCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:793
LEAP-Seq percent confirming:99.1652
LEAP-Seq n confirming:2851
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCCTCCTCCTCTGAGTC
Suggested primer 2:CACCACAGGTTCTGCTTTGA