Insertion junction: LMJ.RY0402.218113_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.218113
Chromosome:chromosome 12
Location:2619062
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g504900 (1 of 13) PF00646 - F-box domain (F-box) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGGACGCGGCCAGCGAATGCAAAAGCC
Internal bar code:CGACGAGCGCTTAGACCATCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:97.212
LEAP-Seq n confirming:1325
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGATGACTGTATTTTGC
Suggested primer 2:CATGGTGAGAGGTTGCAATG