Insertion junction: LMJ.RY0402.218137_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.218137
Chromosome:chromosome 1
Location:2221037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g012150 FKB16-10,MSRA4 Peptide methionine-S-sulfoxide reductase, msrA-type; (1 of 1) 1.8.4.11//5.2.1.8 - Peptide-methionine (S)-S-oxide reductase / Peptide methionine sulfoxide reductase // Peptidylprolyl isomerase / Rotamase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACAAACAAACTCTTCCTCTAGCCACAT
Internal bar code:TGTGTCTGGGTGTATTAGTAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:709
LEAP-Seq percent confirming:99.2784
LEAP-Seq n confirming:1926
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTGAGCCCATCAACAACT
Suggested primer 2:GTCGGCTGTATTTCCCTTGA