| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218154 |
| Chromosome: | chromosome 6 |
| Location: | 8555201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308300 | RRM6 | (1 of 2) IPR002877//IPR029063 - Ribosomal RNA methyltransferase FtsJ domain // S-adenosyl-L-methionine-dependent methyltransferase; Putative ribosomal RNA methyltransferase of the RrmJ/FtsJ family | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGACAGCTGAGCAGCGATAGATAGGC |
| Internal bar code: | TTCTATTTTTTCTGATATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 98.5828 |
| LEAP-Seq n confirming: | 1739 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTTGTAGGTCAGGAGG |
| Suggested primer 2: | ACGGCCCCTTCTGAATAAGT |