| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218183 |
| Chromosome: | chromosome 6 |
| Location: | 3285643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277100 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAATTAGTTCCACAGGCTAACTGTGTCA |
| Internal bar code: | GTGCTGTAAAGCTAGACTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.6377 |
| LEAP-Seq n confirming: | 825 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATCCAGCCAGGGTTGTG |
| Suggested primer 2: | GGTGATGTGGTTGCAGTGTC |