| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218190 |
| Chromosome: | chromosome 4 |
| Location: | 3309507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227000 | MLH2 | DNA mismatch repair protein; (1 of 1) K10858 - DNA mismatch repair protein PMS2 (PMS2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGATGCGGGTGCGTGGGGCTGGCTGAGA |
| Internal bar code: | CTAGGTCTAGCCAATATCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 99.4798 |
| LEAP-Seq n confirming: | 2295 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAAAGGTGGTCTTCTCG |
| Suggested primer 2: | GACTTCATGGAGGTGGAGGA |