| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218193 |
| Chromosome: | chromosome 17 |
| Location: | 676886 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700700 | (1 of 37) IPR008979 - Galactose-binding domain-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCGGCAACGCTCGCCATGGAGCAGGG |
| Internal bar code: | GATCCGCCGGAAGAGAAGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 797 |
| LEAP-Seq percent confirming: | 95.4161 |
| LEAP-Seq n confirming: | 1353 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTTCTACTCACCAGCCG |
| Suggested primer 2: | ACTGACACACGAAGCACAGG |