| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218206 |
| Chromosome: | chromosome 1 |
| Location: | 5904790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042050 | MSRB3 | Methionine sulphoxide reductase; (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGAGCCCCACCTGCTCCGCCATCCCCC |
| Internal bar code: | GGCACGGCTCTGTTGAAGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 63.5593 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 301 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGTGCTGCTGCTACTGC |
| Suggested primer 2: | GACCGCAACGTATTCCTGTT |