| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218234 |
| Chromosome: | chromosome 12 |
| Location: | 4813922 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524500 | RMT2 | Putative protein N-methyltransferase; (1 of 2) PTHR13271//PTHR13271:SF30 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCATGCGGCATTCGTGCGGCACTTGCA |
| Internal bar code: | GGCGCGTCGAAACAAACAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.1195 |
| LEAP-Seq n confirming: | 788 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACTCCCATTGCCAAACG |
| Suggested primer 2: | TGAAAGTTCAGTGCAAACGC |