| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218238 |
| Chromosome: | chromosome 8 |
| Location: | 3737601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377150 | (1 of 1) 2.8.3.22 - Succinyl-CoA--L-malate CoA-transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCCAACTTCGTCTTTGGCAATGGTGCA |
| Internal bar code: | ACCTTACCCACGCAATATTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 99.278 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCGGTAGGTATCGGTAA |
| Suggested primer 2: | CTCAGCTGGAGAGCTGTGTG |