Insertion junction: LMJ.RY0402.218239_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.218239
Chromosome:chromosome 9
Location:2204310
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g393000 CSR6 (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase; Carbohydrate sulfotransferase-related 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCGCGGTGACAGCGTCCATGGCAGAGC
Internal bar code:AGCTGAGCGCGCTTTCCGCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:783
LEAP-Seq percent confirming:99.276
LEAP-Seq n confirming:3565
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACGCTGTGGAACAACAT
Suggested primer 2:GGTTTGCTGCCATTATCCAT