Insertion junction: LMJ.RY0402.218246_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.218246
Chromosome:chromosome 9
Location:4204712
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g394251 PF16 Central pair associated protein; (1 of 1) PF00514//PF13513 - Armadillo/beta-catenin-like repeat (Arm) // HEAT-like repeat (HEAT_EZ) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTTAGCGCCGTTGGGGGCAAAAGCCCT
Internal bar code:TTTTAACGCAGATTTTGGCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:924
LEAP-Seq percent confirming:99.4144
LEAP-Seq n confirming:15788
LEAP-Seq n nonconfirming:93
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGCTTAGCGTCCTGGTT
Suggested primer 2:ACAACTCGAAGTGGTTTGGG