| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218253 |
| Chromosome: | chromosome 3 |
| Location: | 8139197 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200650 | CHI2 | (1 of 1) K17525 - chitinase domain-containing protein 1 (CHID1); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATCCGCCGCCCAGCCAAGTTTCTAACC |
| Internal bar code: | ACGAGTAACCTCCCGTACCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 99.3488 |
| LEAP-Seq n confirming: | 6713 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTTGTCGGGATACATT |
| Suggested primer 2: | ACAGCTTCTGTGTGTGTCCG |