| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218257 |
| Chromosome: | chromosome_3 |
| Location: | 7876982 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202550 | ELG11 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGATAGTGTTTGCCGCCGGCCACATTGCGG |
| Internal bar code: | GCGCTGACTGGCGCTCGTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 99.5551 |
| LEAP-Seq n confirming: | 4252 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGATGTTGAGAAGCAG |
| Suggested primer 2: | CTGACGCTCAAACAGGAACA |