| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218262 |
| Chromosome: | chromosome 8 |
| Location: | 414304 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358579 | (1 of 1) PTHR10984:SF32 - ENDOPLASMIC RETICULUM VESICLE TRANSPORTER PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCTTTGGGCCGTGTTCCAGTGCATATA |
| Internal bar code: | ATTGTGCTACCTGTCTTTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.5432 |
| LEAP-Seq n confirming: | 6538 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGACTTATGGATGTGCC |
| Suggested primer 2: | GCAACCTCAGCTAGTACGGC |