| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218266 |
| Chromosome: | chromosome 5 |
| Location: | 2140635 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234642 | (1 of 16) PF07707 - BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCACGGATGCAATGGGAGAGGGTAAGGG |
| Internal bar code: | GCGGGCCAACGACTCGGTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 99.654 |
| LEAP-Seq n confirming: | 20160 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCATAAAGGAGTGTGCAT |
| Suggested primer 2: | TTTAGTGAGGCGAGGAGCAT |