| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218266 |
| Chromosome: | chromosome 5 |
| Location: | 2140636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234642 | (1 of 16) PF07707 - BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTGTTCCCCATCCTCACCACGCAGCTT |
| Internal bar code: | GTCGGGGCCAATTGTGACTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 97.8428 |
| LEAP-Seq n confirming: | 13834 |
| LEAP-Seq n nonconfirming: | 305 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCATAAAGGAGTGTGCAT |
| Suggested primer 2: | TTTAGTGAGGCGAGGAGCAT |