| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218277 |
| Chromosome: | chromosome 14 |
| Location: | 2746912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627050 | (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCTACAAACCCCCGCACCCCCACGCAC |
| Internal bar code: | ACAGTTCAGACAGGGGATAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.343 |
| LEAP-Seq n confirming: | 6502 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATTGACTTTGCCTGCTGA |
| Suggested primer 2: | ACACACACTCCCTCTCACCC |