| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218282 |
| Chromosome: | chromosome 2 |
| Location: | 2853857 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094750 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTCAAAACGTGGTTCATGAAAGTGAGG |
| Internal bar code: | AGTTTGCACCCTGGCTATCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.6238 |
| LEAP-Seq n confirming: | 15623 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGTGGAGACGGAAGGAA |
| Suggested primer 2: | CAAATGTGTTCAGCCCAATG |