| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218304 |
| Chromosome: | chromosome 4 |
| Location: | 3295538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226900 | (1 of 1) PTHR15441:SF1 - RIBONUCLEASE P PROTEIN SUBUNIT P14 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATCAGCGCCGACGCCATTTCTATCATT |
| Internal bar code: | ATTCCCGTGGATGACCTCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 38.0038 |
| LEAP-Seq n confirming: | 594 |
| LEAP-Seq n nonconfirming: | 969 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACGACCAGTAGCTTGC |
| Suggested primer 2: | GGCTTGGCTAGGACTCACTG |