| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218378 |
| Chromosome: | chromosome 10 |
| Location: | 3863812 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447550 | (1 of 2) PF03200 - Glycosyl hydrolase family 63 C-terminal domain (Glyco_hydro_63) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGGGAAACTTACCCTCGCCACGCCTGC |
| Internal bar code: | GGACGCCGTGGCACGCATGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.7599 |
| LEAP-Seq n confirming: | 3740 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTCCACCAGACCTACC |
| Suggested primer 2: | GGTGCCGCATAAACCACTAT |