| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218389 |
| Chromosome: | chromosome_12 |
| Location: | 3373286 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497200 | FAP216 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGCGACCGTCCGCAAATGCAGCAGCTAA |
| Internal bar code: | CAAACGCTAGAATGGCCGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 91.2254 |
| LEAP-Seq n confirming: | 4720 |
| LEAP-Seq n nonconfirming: | 454 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATGGTCCAAATAGCTCC |
| Suggested primer 2: | GTATGGGACCAAGGCGTAGA |