| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218449 |
| Chromosome: | chromosome 7 |
| Location: | 2196498 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327050 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGAGAAGTGGTCCGCCCCCATGCAG |
| Internal bar code: | AGTCGTAGTGCCTTTCCATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 99.1698 |
| LEAP-Seq n confirming: | 1314 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTTTAAAGCTCCGCAGT |
| Suggested primer 2: | ACCTGAACTCGGTGATGGAG |