| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218454 |
| Chromosome: | chromosome 12 |
| Location: | 1484811 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487500 | CGL61,NYE1, SGR1 | Chlorophyll a dechelatase; (1 of 1) PTHR31750//PTHR31750:SF4 - FAMILY NOT NAMED // PROTEIN STAY-GREEN 1, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGAAGAGCCATGTTTTGTGTGCGCAC |
| Internal bar code: | TACGGCGAATGACGATGGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.6377 |
| LEAP-Seq n confirming: | 2200 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCTCGGTGGAGTTCAGC |
| Suggested primer 2: | CTGGCTAGCTGGTGGAAAAG |