| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218462 |
| Chromosome: | chromosome 7 |
| Location: | 3387669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335100 | (1 of 5) IPR011051//IPR013096 - RmlC-like cupin domain // Cupin 2, conserved barrel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTAAGGCGGTGCCGACTTGTAGCCA |
| Internal bar code: | CCGAGCGTCTAGATCCGTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 98.6301 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCGGCATCAGGTGAGTT |
| Suggested primer 2: | ACCATGAGAAACAAGGCACC |