| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218468 |
| Chromosome: | chromosome 17 |
| Location: | 4607333 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733100 | (1 of 1) IPR000104//IPR003882 - Antifreeze protein, type I // Pistil-specific extensin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCACACGTGTCACAGAATAGATATGGTC |
| Internal bar code: | AGGTCGGATCTGGTTGTACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.586 |
| LEAP-Seq n confirming: | 3849 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGAAAATCTGTGGGGT |
| Suggested primer 2: | GGATTCAGGAGGCATTCAAA |