Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.218469 |
Chromosome: | chromosome_17 |
Location: | 1043499 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g703600 | OFD1 | Basal body protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCGCTCGCCGTGCAGGCGGTCCAGCTCCA |
Internal bar code: | CCGGGCAAGGTCATCGGCCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 962 |
LEAP-Seq percent confirming: | 97.3052 |
LEAP-Seq n confirming: | 1336 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCTTGCTGCAGTAGAATC |
Suggested primer 2: | TATGACAAAGCAGCGCAATC |