| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218478 |
| Chromosome: | chromosome 17 |
| Location: | 5985244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740550 | GT90-33,GT90F33 | GT90 family protein 33; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATTTGCTCCTCCAACACTTGCCGCAGCG |
| Internal bar code: | TCTGTATCCTTTAGGTGATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.6095 |
| LEAP-Seq n confirming: | 3316 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGGATGGTCTGGTCGTT |
| Suggested primer 2: | CAGTAGCTCAGGCCAAGGTC |