| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218483 |
| Chromosome: | chromosome 9 |
| Location: | 4254185 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394510 | (1 of 1) IPR002044//IPR010989//IPR013784 - Carbohydrate binding module family 20 // t-SNARE // Carbohydrate-binding-like fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGGGAACAGAGCTTGGGAGTGGGGCC |
| Internal bar code: | AGACCGATATCTGGTTGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 99.793 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCGCGTTGTTGTACTTC |
| Suggested primer 2: | CATTCCACAACGCTTACACG |