| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218495 |
| Chromosome: | chromosome 13 |
| Location: | 5069555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607100 | CYG6 | (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAGTATGCGGGGGGTACAGCTGCCGCA |
| Internal bar code: | GACGTGGGGTAAGTCGGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 98.6719 |
| LEAP-Seq n confirming: | 3269 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACCAGTCCATCCAGTT |
| Suggested primer 2: | TGTCCACATCACCTGTTGCT |