| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218496 |
| Chromosome: | chromosome 7 |
| Location: | 1592046 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324866 | (1 of 2) IPR000104//IPR000719//IPR001245//IPR011009 - Antifreeze protein, type I // Protein kinase domain // Serine-threonine/tyrosine-protein kinase catalytic domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGAAGTTGCACTTGCGTGTGCGACCC |
| Internal bar code: | GAGGCTGGACGGTTATCAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 99.5044 |
| LEAP-Seq n confirming: | 5019 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACGTGACGTTTCGAACTA |
| Suggested primer 2: | TCAGCTTACAACATGGGCAG |