| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218502 |
| Chromosome: | chromosome 2 |
| Location: | 4672231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101500 | MRS1 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTTGCACACTCGTTGCATGGGTGTTAT |
| Internal bar code: | TCCGTTACAGGCTTTACCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 98.0043 |
| LEAP-Seq n confirming: | 5893 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGACGTTTCATCAGGA |
| Suggested primer 2: | CTTGTTTGTTCCGGTGGACT |