| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218502 |
| Chromosome: | chromosome 2 |
| Location: | 4672233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g101500 | MRS1 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTAAAGAATTCATGCGTTCTCCTTTGTC |
| Internal bar code: | TCAGGCCACGATGCATGTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 97.3008 |
| LEAP-Seq n confirming: | 10454 |
| LEAP-Seq n nonconfirming: | 290 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGACGTTTCATCAGGA |
| Suggested primer 2: | CTTGTTTGTTCCGGTGGACT |