| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218505 |
| Chromosome: | chromosome 9 |
| Location: | 4195786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394139 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACGCAAATTCTTGTCGTCTCAGCGCCT |
| Internal bar code: | TTCTGGGTCCCACCCCATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 97.0571 |
| LEAP-Seq n confirming: | 1682 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCCCACTCTTATGCCCG |
| Suggested primer 2: | TACCTGCAGCAAGCAACAAC |