| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218520 |
| Chromosome: | chromosome 3 |
| Location: | 3800688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170300 | SUB5 | (1 of 1) 3.4.24.30 - Coccolysin / Streptococcus thermophilus intracellular proteinase; Subtilisin-like protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAATTTCAGCGGATTCTTTCTAGAGGA |
| Internal bar code: | CGTGGTTCGGGAAGGTGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 99.8169 |
| LEAP-Seq n confirming: | 3817 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACGGCTACTGCTGCTA |
| Suggested primer 2: | GAATACCCGCAGTGACTCGT |